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. Author manuscript; available in PMC: 2017 Mar 9.
Published in final edited form as: Cell Host Microbe. 2016 Mar 9;19(3):336–348. doi: 10.1016/j.chom.2016.02.004

Figure 5.

Figure 5

Co-localization of endogenous ALIX with Gag at HIV-1 budding sites at the PM requires NC. COS-1 cells expressing WT Gag (panel a, low-power view, and panels b and c, high-power view of other fields), GagΔp2-NCp1-p6 (panels d low-power view, and panels e and f high-power view of other fields) or GagΔp1-p6 (panels g low-power view, and h and i high-power view of other fields) were processed by immunogold double labeling with an antibody to Gag (6-nm gold; indicated by white arrowheads) and cellular ALIX (15-nm gold; indicated by black arrows). Scale bars correspond to 200 nm (panels g, h, i) or 500 nm (a, b, c, d, e, f); (PM) to PM and (Cyt) to cytoplasm. Gag-ALIX colocalization was evaluated by counting gold particles of ~ 300 assembly events at the PM (J).