Extended Data Table 1.
X-ray crystallography data collection and refinement statistics
| FtmOxl | FtmOxl•α-KG Complex | FtmOxl•fumitre morgin B complex | ||
|---|---|---|---|---|
| Data collection | Set-Met | Native | ||
| Space group | P 1 21 1 | P 1 21 1 | P 1 21 1 | P 1 21 1 |
| Cell dimensions | ||||
| a, b, c (Å) | 60.6, 45.8, 105.2 | 60.4, 45.6, 105.4 | 60.6, 45.8, 105.4 | 60.3, 45.4, 104.8 |
| α, β, γ (°) | 90.0, 100.5, 90.0 | 90.0, 99.7, 90.0 | 90.0, 100.0, 90.0 | 90.0, 100.3, 90.0 |
| Resolution (Å) | 48.23 - 3.49 (3.63 - 3.51)* | 42.91 - 1.95 (2.02 - 1.95) | 36.13 - 2.54 (2.63 - 2.54) | 36.05 - 2.11 (2.19 - 2.11) |
| R sym | 0.114 (0.157) | 0.101 (0.725) | 0.125 (0.691) | 0.077 (0.338) |
| I/σI | 13.19 (7.64) | 18.33 (2.02) | 10.53 (1.50) | 17.08 (2.80) |
| Completeness (%) | 99.90 (100.00) | 99.90 (99.22) | 99.93 (99.35) | 99.86 (98.63) |
| Redundancy | 6.1 (3.2) | 6.6 (5.7) | 3.7 (3.7) | 3.7 (3.1) |
| Refinement | ||||
| Resolution (Å) | 42.91 - 1.95 (2.02 - 1.95) | 36.13 - 2.54 (2.63 - 2.54) | 36.05 - 2.11 (2.19 - 2.11) | |
| No. reflections | 41711 | 18951 | 32435 | |
| Rwork/Rfree | 0.1643/0.2043 | 0.1756/0.2340 | 0.1670/0.2033 | |
| No. atoms | 4906 | 4704 | 4884 | |
| Protein | 4535 | 4535 | 4535 | |
| Ligand/ion | 25 | 23 | 38 | |
| Water | 346 | 146 | 311 | |
| B-factors (Å2) | 29.5 | 35.8 | 32.4 | |
| Protein | 29.2 | 35.8 | 31.9 | |
| Ligand/ion | 47.5 | 41.0 | 49.6 | |
| Water | 32.0 | 35.3 | 37.2 | |
| R.m.s deviations | ||||
| Bond lengths (Å) | 0.005 | 0.017 | 0.004 | |
| Bond angles (°) | 0.97 | 0.93 | 0.87 | |
*
Highest resolution shell is shown in parenthesis.