. Author manuscript; available in PMC: 2016 Nov 20.

Published in final edited form as: J Mol Biol. 2015 Jun 26;427(23):3695–3708. doi: 10.1016/j.jmb.2015.06.014

Table 2.

Primers used in this study

Primers	Sequence (5’ to 3’)
for B. subtilis constructs
oRGM2	TACCGTTCGTATAATGTATGCTATACGAAGTTATAGATCAATTTGATAATTACTAATAC
oRGM7	TACCGTTCGTATAGCATACATTATACGAAGTTATTAGAGCTTGGGTTACAGGCATGG
oTB78	GATCCTCGAGAAGCCGGCACGCCATTAAG
oTB79	TTATCGGCCAAGGGAAAC
oTB80	GATCGGATCCAAACCGGTCTGCCATACG
oTB81	CACGGAGCTCATGGGAATGGCCGTACATC
oTB82	GGCCGAATTTATGAAGAGAC
oTB83	AAGATCCGCATTGCTTATGG
for P. aeruginosa constructs
ΔflgK 5’ flank-1	GGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTAGGTGATGAAGTCGCTGGTC
ΔflgK 5’ flank-2	ACTGACCCATGTCCGACCTACCAACCTGATCCAGTTCCAG
ΔflgK 3’ flank-1	CTGGAACTGGATCAGGTTGGTAGGTCGGACATGGGTCAGT
ΔflgK 3’ flank-2	CCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATCACCAAGCAGTACCAGGACA
ΔpilB 5’ flank-1	GGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTTGCCATCCTCCTGCTATTTC
ΔpilB 5’ flank-2	GCAGATCGTTGAAGCCTTCTGCCTTTTCATCGAGAAGTTCA
ΔpilB 3’ flank-1	TGAACTTCTCGATGAAAAGGCAGAAGGCTTCAACGATCTGC
ΔpilB 3’ flank-2	CCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATGCTGGACACGTCTTGTTTGA