Table 1.
List of antibodies targeting components of active centromere. Antibodies were first tested on non-fixative treated cells to determine optimal concentration or dilution for immunocytochemistry and then tested on cells stored in methanol-acetic acid fixative
| Antibody | Species | Concentration or dilution | Cells stored in methanol-acetic acid | |
|---|---|---|---|---|
| 1 | BubR1 [EPR12259(2)] ab183496 (Abcam) | Rabbit IgG monoclonal | 0.527 μg/ml | No |
| 2 | CENP-A (Clone: 3–19) | Mouse IgG monoclonal | 1 : 500 | No |
| 3 | CENP-A phospho Ser18 (Active Motif) | Rabbit IgG polyclonal | 1 : 400 | No |
| 4 | CENP-C (Serum from rabbit 554; gift from William Earnshaw) | Rabbit polyclonal | 1 : 1000 | Yes |
| 5 | CENP-C [EPR15939] ab193666 (Abcam) | Rabbit IgG monoclonal | 0.677 μg/ml | Yes |
| 6 | CENP-E [EPR4542(2)] ab133583 (Abcam) | Rabbit IgG monoclonal | 0.275 μg/ml | Not homogeneous across all chromosomes |
| 7 | HEC1 [9G3] ab3613 (Abcam) | Mouse IgG monoclonal | 0.5 μg/ml | No |