Table 4.
mRNA expression after TNF stimulation in human umbilical vein endothelial cell
| 0 min | 15 min | 30 min | 1 h | 4 h | 8 h | |
|---|---|---|---|---|---|---|
| ATG5 | 1 | 0.5 | 0.5 | 0.7 | 0.3 | 0.1 |
| ATG8 | 1 | 2.3 | 1.8 | 2.6 | 2.1 | 0.3 |
| ATG12 | 1 | 2.2 | 3.9 | 3.1 | 2.2 | 0.8 |
| c-FOS | 1 | 13.6 | 22.9 | 5.3 | 2.8 | 1.3 |
| c-Jun | 1 | 4.6 | 12.5 | 4.9 | 1.1 | 0.8 |
| ERK1 | 1 | 1.2 | 0.8 | 0.7 | 1.0 | 0.5 |
| FADD | 1 | 2.2 | 3.1 | 3.8 | 1.8 | 1.1 |
| ILAK1 | 1 | 8.8 | 14.2 | 12.4 | 11.0 | 3.9 |
| ILAK2 | 1 | 2.1 | 1.8 | 1.6 | 1.2 | 0.7 |
| ILAK4 | 1 | 2.2 | 1.8 | 1.6 | 1.4 | 0.5 |
| MAPK14 | 1 | 4.2 | 3.4 | 3.4 | 3.9 | 1.5 |
| MyD88 | 1 | 2.2 | 2.1 | 1.7 | 2.1 | 0.3 |
| p62 | 1 | 1.3 | 3.5 | 9.4 | 7.7 | 1.6 |
| p65 | 1 | 0.8 | 1.4 | 1.4 | 1.1 | 0.5 |
| RIPK | 1 | 3.2 | 2.7 | 2.2 | 3.4 | 0.8 |
| STAT3 | 1 | 6.3 | 2.4 | 2.8 | 3.0 | 0.2 |
| TLR2 | 1 | 6.1 | 2.8 | 5.3 | 2.6 | 0.4 |
| TLR4 | 1 | 1.6 | 1.3 | 1.7 | 1.5 | 0.2 |
| TAK1 | 1 | 2.8 | 3.9 | 3.1 | 3.6 | 0.8 |
| TNFRSF1A | 1 | 12.4 | 12.1 | 12.2 | 12.3 | 3.1 |
| ULK1 | 1 | 3.7 | 4.7 | 2.9 | 5.1 | 1.8 |
Human umbilical vein endothelial cells (HUVEC) were observed in the time course in a separate medium after TNFα 100 ng/mL administration without steroid and the other anti-inflammatory molecules. At 8 h after TNFα stimulation, the endothelial cells were observed with shape change and cell death in approximately 32 %. High expression of mRNA of ILAL1, p62, TNFRF1A, and ULK were observed in HUVEC through the time course after TNFα by reverse transcription polymerase chain reaction of the inner control of actin mRNA