TABLE I. Primers sequence, thermal cycling profile, and size of amplified polymerase chain reaction (PCR) fragment in each PCR reaction in the detection of genes of Staphylococcus epidermidisresistant to erythromycina .

Gene	Primers sequence	PCR conditions	PCR fragment size (bp)
ermA	5’-TCTAAAAAGCATGTAAAAGAA-3’ 5’-CTTCGATAGTTTATTAATATTAGT-3’	35 (30 s at 94ºC, 1 min at 48ºC, 2 min at 72ºC)	645
ermB	5’-GAAAAGGTACTCAACCAAATA-3’ 5’-AGTAACGGTACTTAAATTGTTTAC-3’	35 (30 s at 94ºC, 30 s at 50ºC, 2 min at 72ºC)	639
ermC	5’-AGTACAGAGGTGTAATTTCG-3’ 5’-AATTCCTGCATGTTTTAAGG-3’	35 (55 s at 94ºC, 1 min at 53ºC, 1 min at 72ºC)	642
msrA	5’-GGCACAATAAGAGTGTTTAAAGG-3’ 5’-AAGTTATATCATGAATAGATTGTCCTGTT-3’	25 (1 min at 94ºC, 1 min at 50ºC, 90 s at 72ºC)	399
mphC	5’-GAGACTACCAGACCTGACG-3’ 5’-CATACGCCGATTCTCCTGAT-3’	35 (1 min at 94ºC, 1 min at 59ºC, 1 min at 72ºC)	530
linA/A’	5’-GGTGGCTGGGGGGTAGATGTATTAACTGG-3’ 5’-GCTTCTTTTGAAATACATGGTATTTTTCGATC-3’	30 (30 s at 94ºC, 30 s at 57ºC, 1 min at 72ºC)	323

a: Sutcliffe et al. (1996) and Lina et al. (1999).