TABLE II. Susceptibility profile and point mutations found in theERG11 gene coding region of clinical isolates of the Candida genus.
| Species | Isolate | GenBank | Isolation site | MIC (μg mL-1) | Point mutations | ||
|---|---|---|---|---|---|---|---|
|
| |||||||
| Fluconazole | Itraconazole | Voriconazole | |||||
| C. glabrata (1,603 bp) | 90030 | KR998002 | ATCC | (S) | (S) | (S) | A1581Ga |
| HU10 | KR998003 | Uroculture | 8 (S) | ≤ 0.125 (S) | 1 (-) | C423Ta, T768C, T1557A, A1581Ga | |
| HU25 | KR998004 | Uroculture | 8 (S) | ≥ 1 (R) | 1 (-) | C201G, T768C, A1023G, T1275C, T1557A, A1581Ga | |
| HU26 | KR998005 | Uroculture | 8 (S) | ≥ 1 (R) | ≥ 4 (-) | T768C, G927A, A1023G, T1557A, A1581Ga | |
| HU33 | KR998006 | Rectal swab | 8 (S) | ≤ 0.125 (S) | ≥ 4 (-) | T768C, A1023G, T1557A, A1581Ga | |
| HU37 | KR998007 | Uroculture | 8 (S) | ≤ 0.125 (S) | ≥ 4 (-) | C423Ta, T768C, A1023G, T1275C, T1557A, A1581Ga | |
| HU40 | KR998008 | Haemoculture | ≥ 64 (R) | ≥ 1 (R) | ≥ 4 (-) | T768C, G927A, A1023G, T1275C, T1557A, A1581Ga | |
| HU61 | KR998009 | Uroculture | 16 (S) | ≥ 1 (R) | 1 (-) | C423Ta, T768C, A1023G, T1275C, T1557A, A1581Ga | |
| HU66 | KR998010 | Uroculture | 16 (S) | ≤ 0.125 (S) | ≥ 4 (-) | C108Ga, C423Ta, T768C, A1023G, T1275C, T1557A, A1581Ga | |
| C. krusei(1,607 bp) | 6258 | KR998011 | ATCC | (S) | (S) | (S) | T1389C, A1470C, G1570Ab |
| HU45 | KR998012 | Rectal swab | 8 (-) | ≤ 0.125 (S) | 1 (DDS) | T642C, A756T, T1389C, A1470C, G1570Ab | |
| HU11 | KR998013 | Nasal swab | ≥ 64 (-) | ≤ 0.125 (S) | 1 (DDS) | T642C, A756T, T1389C, A1470C, G1570Ab | |
| HU18 | KR998014 | Haemoculture | ≥ 64 (-) | ≤ 0.125 (S) | 1 (DDS) | A497Cb, T642C, T1389C, A1470C, G1570Ab | |
| C. tropicalis (1,587 bp) | 750 | KR998015 | ATCC | N | |||
| HU80 | KR998016 | Uroculture | 8 (R) | ≤ 0.125 (S) | ≥ 4 (R) | T783C | |
| HU48 | KR998017 | Catheter tip | 16 (R) | ≤ 0.125 (S) | 1 (R) | G1362A, T1554 | |
| HU54 | KR998018 | Uroculture | 8 (R) | ≤ 0.125 (S) | 1 (R) | G1362A | |
a: new synonymous mutation; b: new nonsynonymous mutations, A497C = Y166S and G1570A = G524R; DDS: dose dependent sensitivity; MIC: minimum inhibitory concentration; N: absent base substitution; R: resistant; S: sensitive; -: no significant evidence to determine the cut-off value for the species.