Table 1.
Bacterial strains and plasmids
| Name | Description | Reference |
|---|---|---|
| Strains | ||
| E. coli DH5α | F− ϕ80ΔlacZM15 Δ(lacZYAargF)U169 deoP recA1 endA1 hsdR17 (rk − mk −) λ− | Gibco BRL |
| E. coli TOP10F’ | F’ {lacIq Tn10(TetR)} mcrA ∆(mrr-hsdRMS-mcrBC) φ80lacZ∆M15 ∆lacX74 deoR recA1 araD139 ∆(ara-leu)7697 galU galK rpsL (StrR) endA1 nupG | Invitrogen |
| S. Typhimurium SL1344 | Wildtype strain, xyl hisG rpsL; virulent; SmR | [82] |
| CMPG5624 | S. Typhimurium SL1344, ΔfabR | This study |
| CMPG5825 | S. Typhimurium SL1344, fabR-M9 | This study |
| Plasmids | ||
| c3390 | SpeI-myc9-SpeI cassette in pBlueskript SKII- | [39] |
| pCP20 | flp, ts-rep-[ciI857](λ) ts, ApR, CmR | [38] |
| pCMPG5553 | yqfA cloned XbaI/SacI in pFAJ1708 | This study |
| pCMPG5678 | fabR cloned EcoRI/BamHI in pFAJ1708 | This study |
| pCMPG10118 | fabA cloned XbaI/EcoRI in pFAJ1708 | This study |
| pCMPG10119 | fabB cloned XbaI/EcoRI in pFAJ1708 | This study |
| pFPV25 | Promoter-trap vector constructed by inserting an EcoRI-HindIII fragment containing a promoterless gfpmut3 [83] into plasmid pED350 (colE1, bla, mob) [84]; ApR | [85] |
| pKD3 | Plasmid used as template for construction of Salmonella mutants; ApR, CmR | [38] |
| pKD46 | Lambda Red helper plasmid, ApR | [38] |