Table 4.
ANOVA for the quadratic model for CMCase activity and protease activity of B. subtilis IND19
| Source | Sum of squares | df | Mean square | F value | p value | |
|---|---|---|---|---|---|---|
| CMCase activity of B. subtilis IND19 | ||||||
| Model | 2.75E+05 | 9 | 3.05E+04 | 67.14 | <0.0001 | Significant |
| A-pH | 5.27E+04 | 1 | 5.27E+04 | 116.04 | <0.0001 | |
| B-Peptone | 4.46E+04 | 1 | 4.46E+04 | 98.04 | <0.0001 | |
| C-NaH2PO4 | 3.26E+04 | 1 | 3.26E+04 | 71.81 | <0.0001 | |
| AB | 9.25E+03 | 1 | 9.25E+03 | 20.35 | 0.0011 | |
| AC | 2.88E+02 | 1 | 2.88E+02 | 0.63 | 0.4445 | |
| BC | 924 | 1 | 924 | 2.03 | 0.1843 | |
| A 2 | 69,800.17 | 1 | 69,800.17 | 153.57 | <0.0001 | |
| B 2 | 1.89E+04 | 1 | 1.89E+04 | 41.51 | <0.0001 | |
| C 2 | 6.87E+04 | 1 | 6.87E+04 | 151.24 | <0.0001 | |
| Residual | 4.55E+03 | 10 | 454.51 | |||
| Lack of fit | 3456.22 | 5 | 691.24 | 3.17 | Not significant | |
| Pure error | 1.09E+03 | 5 | 217.77 | |||
| Cor total | 2.79E+05 | 19 | ||||
| Protease activity of B. subtilis IND19 | ||||||
| Model | 2.95E+09 | 9 | 3.27E+06 | 197.54 | <0.0001 | Significant |
| A-pH | 2.30E+04 | 1 | 2.30E+04 | 1.39 | 0.266 | |
| B-Peptone | 4.82E+0.005 | 1 | 4.82E+0.005 | 291.22 | <0.0001 | |
| C-NaH2PO4 | 7.02E+05 | 1 | 7.02E+05 | 42.35 | <0.0001 | |
| AB | 2.412E+0.05 | 1 | 2.412E+0.05 | 14.55 | 0.0034 | |
| AC | 2.12E+06 | 1 | 2.12E+06 | 127.58 | <0.0001 | |
| BC | 8.70E+06 | 1 | 8.70E+06 | 524.67 | <0.0001 | |
| A 2 | 1.11E+07 | 1 | 1.11E+07 | 670.76 | <0.0001 | |
| B 2 | 2.31E+06 | 1 | 2.31E+06 | 139.47 | <0.0001 | |
| C 2 | 1.43E+04 | 1 | 1.43E+04 | 0.86 | 0.3744 | |
| Residual | 1.66E+05 | 10 | 16,575.16 | |||
| Lack of fit | 23,264.15 | 5 | 4652.83 | 0.16 | 0.9658 | Not significant |
| Pure error | 1.43E+05 | 5 | 28,497.5 | |||
| Cor total | 2.96E+07 | 19 | ||||