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. 2016 Mar 23;16:55. doi: 10.1186/s12866-016-0655-1

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© Granato et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Comparison of type IV pili gene expression (using qRT-PCR) in the wild-type and the ∆hrpB bacteria that were cultured in NB medium and in planta. a Relative expression levels on NB medium. b Relative expression levels in planta. The data are presented as the ratio (Log₂-fold change) of the transcript number in ∆hrpB compared to the number in wild-type. The DNA gyrase subunit A-encoding gene gyrA was used as an endogenous control. Both qRT-PCR assays were repeated twice with similar results, and three independent biological replicates were performed each time