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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Biochim Biophys Acta. 2015 Dec 18;1861(3):196–204. doi: 10.1016/j.bbalip.2015.12.006

Fig. 3.

Fig. 3

Sterol secretion and bile acid formation in Huh7 cells. Cells were pulsed for 2 h with HDL-, CERM- or LDL-[14C]CE at 32–61 nmol CE/mL media, washed, and harvested (2 h) or chased in fresh media and harvested at 4, 6 or 8 h. Chase media was collected for analysis of secreted C-sterols. Cell lipids and media were partitioned to determine the ratios of bile acids to neutral sterols. A. Secreted sterol as a fraction of the total sterol in cells plus media. B. Bile acid percent of the secreted 14C-sterols. C. Bile acid percent of the cell 14C- sterols. HDL and CERM were tested at 2, 4 and 8 h, LDL was tested only at 4 h. Asterisks indicate significant differences, p b 0.05, between the bracketed groups.