Table 2. Lipid content of Huh7 cells.
| Harvest Density, 104 cells/cm2 | 3.4 | 21.0 | pb |
|---|---|---|---|
| Lipidsa | nmoles/mg cell protein, mean ± SD | ||
| Total cholesterol | 91 ± 6 | 150 ± 15 | 0.007 |
| Cholesteryl Ester | 16 ± 4 | 30 ± 8 | 0.088 |
| Free Cholesterol | 75 ± 3 | 120 ± 7 | 0.001 |
| Triglyceride | 80 ± 8 | 132 ± 4 | 0.000 |
| Phosphatidyl Choline | 132 ± 2 | 179 ± 18 | 0.026 |
| Oxysterols | pmoles/mg cell protein, mean ± SD | ||
| 27-Hydroxycholesterol | 379 ± 87 | ||
| 5β,6β-Epoxycholestanol | 239 ± 92 | ||
| 7α-Hydroxycholesterol | 228 ± 51 | ||
| 7β-Hydroxycholesterol | 106 ± 36 | ||
| 4β-Hydroxycholesterol | 74 ± 23 | ||
| 25-Hydroxycholesterol | 59 ± 25 | ||
| 24(S)-Hydroxycholesterol | 26 ± 9 | ||
| 7-Ketocholesterol | 26 ± 10 | ||
| 5α,6α-Epoxycholestanol | 13 ± 8 | ||
| 3β,5α,6β-Trihydroxycholestane | 7 ± 5 | ||
| Total oxysterols | 1157 ± 273 | ||
| Bile Acidsc | pmoles/mg cell protein, mean ± SD | ||
| Cholate | 0.20 ± 0.10 | ||
| Chenodeoxycholate | 0.26 ± 0.08 | ||
| Total bile acids | 0.46 ± 0.14 | ||
a
Two (low density) or four (high density) dishes of Huh7 cells were harvested for lipid and protein assays. Oxysterols and bile acids were determined for the high density harvested cells. Each assay was done in triplicate.
b
Student t-test p value comparing lipid content at high and low density.
c
Glycocholate, taurocholate, glycochenodeoxycholate and taurochenodeoxycholate were not detected in the cell samples.