Fig. 6.

Using this single cell-handling approach, different tissue sizes and geometries of 3D-dhECTs can be formed. Uniformly contracting tissues, in addition to (A) immobilized microislands, included: (B) macrotissues and (C) microspheres, all of which were able to be used to obtain (D) 3D-dhECT-dissociated single CMs. (E) Tissue sizes and geometries can be varied based on desired future applications, e.g., microislands for high-throughput drug-screening, macrotissues for large-scale mechanical testing and cardiac patches, injectable cardiac tissue spheroids, or single CMs post-tissue digestion for automated patch clamping.