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. Author manuscript; available in PMC: 2017 Mar 5.
Published in final edited form as: Eur J Pharmacol. 2016 Feb 3;774:25–33. doi: 10.1016/j.ejphar.2016.01.007

Fig 2. Determination of optimal substrate concentration for ACE and ACE2 activity assays.

Fig 2

Rate of hydrolysis was evaluated over a range of substrate concentrations in the presence of recombinant enzymes or human or mouse cell lysates with ACE- or ACE2-specific substrates. Shown were representatives of four repetitions (recombinant enzymes) or experiments carried out in four different samples (human or murine cells). (A) rhACE2 (B) human MNC ACE2 (C) murine MNC ACE2 (D) rhACE (E) human MNC ACE and (F) murine MNC ACE.