. Author manuscript; available in PMC: 2017 Mar 5.

Published in final edited form as: Eur J Pharmacol. 2016 Feb 3;774:25–33. doi: 10.1016/j.ejphar.2016.01.007

Table 2. Dissociation constant K_i and IC₅₀ values of antagonists, isomer B of MLN4760 and DX600, in different samples.

	MLN4760 (Isomer B)		DX600

Enzyme	K_i (μM)	IC₅₀ (μM)	K_i (μM)	IC₅₀ (μM)
Recombinant Human ACE2	0.04 ± 0.005	0.01 ± 0.006	0.02 ± 0.008	0.01 ± 0.003
Human ACE2	0.2 ± 0.01	0.5 ± 0.03	0.2 ± 0.01	0.3 ± 0.05
Murine ACE2	2.1 ± 0.2	7.1 ± 0.63	1.2 ± 0.9	0.4 ± 0.07

Ki values were determined as described in the text. Assays were repeated four times in case of recombinant ACE2 or carried out in four different samples in case of human and murine cells. IC₅₀ values were obtained from current study as described in the results section.

Table 2. Dissociation constant Ki and IC50 values of antagonists, isomer B of MLN4760 and DX600, in different samples.

Table 2. Dissociation constant K_i and IC₅₀ values of antagonists, isomer B of MLN4760 and DX600, in different samples.