AM3677 elicits CB1R-mediated partial agonist activity when measured as [35S]GTPγS binding to CB1R in mouse hippocampus. In these studies, the maximum response is defined as the response produced by 10 μM CP55,940. (A) AM3677 elicited partial G protein coupling in this measure of response and was more potent than its endocannabinoid analogue, AEA. (B) The response produced by either 3 μM AM 3677, 10 μM CP55,940, or 10 μM anandamide could be completely inhibited by the CB1R competitive antagonist SR14716A in a concentration-dependent manner. Each data point is the mean ± SEM of three to four independent experiments performed in duplicate.