. Author manuscript; available in PMC: 2016 Mar 23.

Published in final edited form as: ACS Chem Neurosci. 2015 May 29;6(8):1400–1410. doi: 10.1021/acschemneuro.5b00090

Table 1.

Apparent AM3677 Binding Affinities of WT hCB1R and Cys Mutants

hCB1R variant	apparent K_i (95% CI; nM)^a
WT	1.7 (0.7–3.8)
C1.55(139)S	2.3 (1.0–5.3)
C4.47(238)S	2.4 (0.8–7.2)
C6.47(355)S	2.2 (0.8–5.8)
C7.38(382)S	1.9 (0.4–8.1)
C7.42(386)S	1.6 (0.5–5.0)

Apparent binding affinities for AM3677 (as K_i values) were derived from competitive binding assays with [³H]CP55,940 and membrane preparations from stably transfected Flp-In cells. Data are the mean of at least three independent experiments carried out in triplicate, with 95% confidence intervals (CIs) shown in parentheses.