| 1 cycle | 98°C | 2 minutes |
| 8, 10, 12 and 16 cycles | 98°C | 10 seconds |
| 60°C | 15 seconds | |
| 72°C | 45 seconds | |
| 1 cycle | 72°C | 5 minutes |
| 1 cycle | 4°C | HOLD |
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