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. 2016 Mar 23;7:33. doi: 10.3389/fgene.2016.00033

Figure 8.

Figure 8

Expression of corresponding TF genes which can be represented by the PWM V$AP1FJ_Q2 have been shown in Figure 4A. (A) Expression of corresponding genes to TFs which can be represented by the PWM V$SMAD_Q6_01. Each SMAD is expressed during the complete time course at similiar levels, while the expression levels of SMAD2/4 are higher than the expression levels of SMAD1/3. After beginning of the cardiac specification (> day 3) SMAD4 is slightly more abundant than SMAD2 and remains in this position. (B) Expression of corresponding TF genes which can be represented by the PWM V$LEF1TCF1_Q4, TCF7 is below the threshold set by us as a limit for robust transcription while LEF1 is clearly transcribed after the mesoderm induction stage (> day 3). The SMAD-TCFLEF-pair was found during the cardiac specification stage only (day 3–day 8). (C) Expression of corresponding TF genes which can be represented by the PWM V$FOX_Q2. FOXH1 is the only expressed gene and present until day 13. The red lines show a FPKM value of 10 that we consider as threshold for sufficiently expressed genes which contribute to regulatory effects.