Fig. 2.

Quantitative TLC ³²P-postlabeling analysis of AAI-DNA adduct levels in organs of rats treated with AAI, Sudan I or AAI after exposure to Sudan I. Numbers above columns (“F”) indicate fold changes in DNA adduct levels in animals treated with AAI combined with Sudan I compared to animals treated with AAI alone. Values are given as the means ± SD (n = 3); each DNA sample was determined by two postlabeled analyses. RAL, relative adduct labeling. Comparison was performed by t-test analysis; ***P < 0.001, different from animals treated with AAI alone. Insert: Autoradiographic profile of AAI-DNA adducts formed in liver of rats treated with AAI, determined by the nuclease P1 enrichment version of the ³²P-postlabeling assay.