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. 2014 May;30(3):215–222.

Figure 4.

Figure 4

GbE increased endothelial NO synthase (eNOS) expression at the RNA and protein level, which subsequently increased nitric oxide (NO) production. HUVECs were treated with DMSO (control) or GbE (10, 50, or 100 μg/mL) for 24 h. Cells were collected and eNOS mRNA and protein expression levels were determined with PCR and Western blot, respectively. eNOS expression levels increased in a dose-dependent manner at the (A) mRNA and (B) protein level, which coincided with increases in (C) NO production, as detected by flow cytometry with 4,5-diaminofluorescein. Data are presented as mean ± SD of experiments performed in triplicate. * p < 0.05 and # p < 0.001 vs. control.