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Parthenolide elicited ROS formation. (A) H9c2 cells were treated with DCFH-DA and then incubated in the absence or presence of parthenolide (30 μM) for 1 h and ROS generation was assayed using flow cytometry. (B) Quantification of results in (A). Results are mean ± standard error of the mean of 4 experiments. * indicates significant difference (p < 0.05) from the control. DCFH-DA, 2,7-dichlorodihydrofluorescein diacetate; FITC, Fluorescein isothiocyanate; Par, parthenolide.
