Figure 3.

(A) Proliferative ability of pulmonary artery smooth muscle cells in the hypoxic and normoxic groups, as detected by EdU assay (magnification, ×100). The number of EdU-positive cells increased as hypoxic exposure time was prolonged. (B) The positive rates were as follows: Normoxia, 6.86±0.25%; hypoxia 2 h, 7.08±0.38%; hypoxia 6 h, 9.11±0.24%; hypoxia 12 h, 29.99±1.48%; hypoxia 24 h, 52.12±0.89%; hypoxia 48 h, 53.69±0.64%. Data are expressed as the mean ± standard deviation, n=5. ^*P<0.05. EdU, 5-ethynyl-2′-deoxyuridine; DAPI, 4′,6-diamidino-2-phenylindole.