Figure 7.

(A-C) mRNA and (D) protein expression levels of (A) SENP-1, (B) HIF-1α and (C) VEGF in pulmonary artery smooth muscle cells with SENP-1 knockdown exposed to hypoxia for 12 h. In the SENP-1 shRNA group, HIF-1α and VEGF exhibited a downregulation at the mRNA and protein level, as compared with the scramble and blank groups under hypoxic conditions. Data are presented as the mean ± standard deviation. ^*P<0.05 vs. the scramble and blank groups. SENP-1, small ubiquitin-like modifier-specific protease 1; HIF-1α, hypoxia-inducible factor-1α; VEGF, vascular endothelial growth factor; shRNA, short hairpin RNA.