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An EdU assay was conducted to determine the proliferation of pulmonary artery smooth muscle cells with SENP-1 knockdown exposed to hypoxia for 12 h (magnification, ×100). In the SENP-1 shRNA group, less EdU-positive cells were detected, as compared with in the scramble and blank groups under hypoxic conditions. *P<0.05. SENP-1, small ubiquitin-like modifier-specific protease 1; EdU, 5-ethynyl-2′-deoxyuridine; DAPI, 4′,6-diamidino-2-phenylindole; shRNA, short hairpin RNA.
