Table I.
Primers used to amplify rat SENP-1, HIF-1α, VEGF and β-actin mRNA, and the associated polymerase chain reaction procedures.
| Target gene | Oligonucleotide primers (5′-3′) | Annealing temperature (°C) | Cycles | Produc size (bp) |
|---|---|---|---|---|
| HIF-1α | Forward TGTGGATAGCGATATGGTCAA | 53.1 | 31 | 218 |
| Reverse CTCTTTCCTGCTCTGTCTGGT | ||||
| SENP-1 | Forward TGCAGTGCTTGATTCCGTAG | 63.1 | 32 | 405 |
| Reverse TTCTTTGTCCAGCGTTTCAC | ||||
| VEGF | Forward CATCCACCATGCACTTGCTGT | 55.8 | 28 | 178 |
| Reverse GGCTGCTCCAAACTCCTTCCA | ||||
| β-actin | Forward AGCCATGTACGTAGCCATCC | 65 | 28 | 228 |
| Reverse CTCTCAGCTGTGGTGGTGAA |
HIF-1α, hypoxia-inducible factor-1α; SENP-1, small ubiquitin-like modifier-specific protease 1; VEGF, vascular endothelial growth factor.