Table I.

Primers used to amplify rat SENP-1, HIF-1α, VEGF and β-actin mRNA, and the associated polymerase chain reaction procedures.

Target gene	Oligonucleotide primers (5′-3′)	Annealing temperature (°C)	Cycles	Produc size (bp)
HIF-1α	Forward TGTGGATAGCGATATGGTCAA	53.1	31	218
	Reverse CTCTTTCCTGCTCTGTCTGGT
SENP-1	Forward TGCAGTGCTTGATTCCGTAG	63.1	32	405
	Reverse TTCTTTGTCCAGCGTTTCAC
VEGF	Forward CATCCACCATGCACTTGCTGT	55.8	28	178
	Reverse GGCTGCTCCAAACTCCTTCCA
β-actin	Forward AGCCATGTACGTAGCCATCC	65	28	228
	Reverse CTCTCAGCTGTGGTGGTGAA

HIF-1α, hypoxia-inducible factor-1α; SENP-1, small ubiquitin-like modifier-specific protease 1; VEGF, vascular endothelial growth factor.