Table 1. MIC50 (μM) of various fungal species.
| MIC50 (μM) | |||||
|---|---|---|---|---|---|
| Media | Test agent | A. fumigatus | C. albicans | C. neoformans | S. cerevisiae |
| Incubation parameters | (24h, 37°C) | (24h, 30°C) | (48h, 37°C) | (24h, 30°C) | |
| RPMI only | AMB suspension | 1.08 | 0.541 | 0.541 | 1.08 |
| AMB-PEG 1 | 8.66 | 4.33 | 4.33 | 4.33 | |
| AMB-PEG 2 | 8.66 | 4.33 | 4.33 | 4.33 | |
| RPMI + 10% FBS | AMB suspension | 0.541 | 0.271 | 0.271 | 0.541 |
| AMB-PEG 1 | 34.6 | 8.66 | 8.66 | 8.66 | |
| AMB-PEG 2 | 17.3 | 4.33 | 4.33 | 4.33 | |
Susceptibility testing was performed using AMB-PEG formulations that have been passed through a 10kDa centrifugal filter and quantified, as well as unconjugated AMB, under stated incubation conditions. AMB-PEG 2 is approximately twice as effective at inhibiting fungal growth in serum-supplemented media. Unconjugated AMB also has better antifungal activity compared to the AMB-PEG formulations, especially in serum-containing media. Results reported are from 3 independent experiments.