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. 2016 Mar 23;12(3):e1005510. doi: 10.1371/journal.ppat.1005510

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© 2016 Sattler et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 5 — Formaldehyde cross-linking ChIP assays were performed to ensure that Hexim1 and Rbbp4 are associated with DNA of the right oriLyt of MHV-68. The immunoprecipitates isolated from TCMK-1 cells by a specific antibody against Hexim1 (A) or from NIH 3T3 cells by a specific antibody against Rbbp4 (B) were analyzed by quantitative PCR with primer pairs designed to amplify a part of the DNA sequence of the right oriLyt and the left oriLyt, respectively, or by a primer pair amplifying a non-relevant sequence located in the viral ORF23.