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. 2016 Mar 23;11(3):e0151329. doi: 10.1371/journal.pone.0151329

Fig 1. JQ1 increases insulin secretion, content and gene expression in pancreatic β-cells.

Fig 1

The rat pancreatic β-cell line INS-1, or isolated islets, were cultured for 3 days as described in Methods, in the presence of indicated concentrations (nM) of JQ1. Except where specified, JQ1 was the active ‘S’ (+) enantiomer [34]. The inactive ‘R’ (-) enantiomer was the negative control. (A) Analysis of insulin secretion in INS-1 cells. Insulin secretion was measured under either basal glucose conditions (2 mM, white bars) or stimulated conditions (8 mM, gray bars). (n = 4) (B) Analysis of insulin content in INS-1 cells. (n = 4) (C) Analysis of insulin secretion, corrected for insulin content, in INS-1 cells. (n = 3) (D) Analysis of Ins-1 gene expression in INS-1 cells, measured by RT-PCR. (n = 2 separate experiments). (E) Analysis of insulin content in rat islets. (n = 3) (F) Analysis of insulin content in human islets. (3 samples from 2 donors; * p<0.05).