Skip to main content
. 2016 Mar 23;11(3):e0151329. doi: 10.1371/journal.pone.0151329

Fig 5. Microarray gene analysis of BET protein specific siRNA treated INS-1 cells.

Fig 5

(A) Brd4 siRNA has the greatest effect to increase Ins1 and Ins2 gene expression whereas Brd2 siRNA treatment has the greatest effect to reduce Acaca gene expression and increase Pdx1 gene expression, compared to NT control. (n = 3 separate experiments). * p<0.05, ** p<0.001. (B) Heatmap of 1100 genes that were significantly differently expressed compared to the NT control. Red and blue bars represent values that are 2 standard deviations above or below the average of each row (shown in white), respectively. Numbered samples were treated as follows: 1–3 with NT control siRNA, 4–6 with Brd2 siRNA, 7–9 with Brd3 siRNA and 10–12 with Brd4 siRNA. Gene identities and hierarchical clustering can be found in S2 File.