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. 2016 Mar 23;11(3):e0152031. doi: 10.1371/journal.pone.0152031

Fig 2. In vivo characterization of 3xIRF-ISRE-Luc reporter plasmid delivered to mouse liver by hydrodynamic injection.

Fig 2

A) Mice received a hydrodynamic injection with 20 μg of 3xIRF-ISRE-Luc reporter plasmid through the tail vein. Once luciferase activity stabilized (one month after injection), mice were treated intraperitoneally with the indicated doses of murine IFN-β. Light emission was quantified by BLI 10 and 24 hours after treatment. Values correspond to fold luciferase activity, using baseline (pre-induction) activity as a reference. B) Quantitative RT-PCR of OAS and Mx1 genes in peripheral blood lymphocytes of animals treated for 24 hours with different doses of recombinant murine IFN-β. C) Reporter activity re-induction in mice determined every week by intraperitoneally administration of 10,000 units of IFN-β. Each line represents an individual mouse. D) Representative BLI images of mice before and 10 hours after administration of 10,000 U of murine IFN-β. These data are from one experiment representative of three.** p<0.01 vs 3,000, 1,000 and 0 IFN-β units.