Fig 5. W1282X-CFTR channels in FRT patches are virtually ‘locked open’ by adding curcumin after a saturating dose of VX-770.
(A) Multichannel record for inside-out patch excised from FRT cell stably expressing W1282X-CFTR. Control conditions were identical to Fig 1. 300 nM VX-770 and 30 μM curcumin were added where indicated. Holding potential = 60 mV. This patch contained 6 channels. The Po estimated for each condition in this experiment is indicated. (B) Higher gain records for the experiment in panel A. (C) Mean Po, single channel opening rate and mean burst duration for W1282X-CFTR channels estimated for the indicated conditions. Error bars are SEs. N’s are indicated in parentheses. *p<0.05 and **<0.01 by paired t-test as indicated.