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. 2016 Mar 23;11(3):e0152232. doi: 10.1371/journal.pone.0152232

Fig 7. Activation of W1282X-CFTR mediated currents in FRT cell monolayers by the combination of VX-770 and curcumin.

Fig 7

(A) Western blot analysis of stably transfected FRT cells. Lane 1: WT-CFTR; lane 2: control FRT cell lysate; lane 3: W1282X- CFTR. The number indicates relative amount of CFTR protein. 40 μg total protein was loaded in each lane. Tubulin controls are shown in the bottom lanes. (B) Short circuit current measurement for FRT cells expressing WT-CFTR (see Experimental Procedures for details). Drug concentrations were 10 μM forskolin, 5 μM VX-770, 40 μM curcumin and 10 μM CFTR(inh)-172, (C) Short circuit measurement for W1282X-CFTR expressing FRT cells. Each trace represents an individual experiment. (D) The bars represent mean (+/- SE) increase in short circuit current above forskolin-stimulated current upon addition of VX-770 and/or curcumin followed by the combination of the two compounds in W1282X-CFTR expressing cells.