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High-resolution mass spectra showing the m/z isotope cluster corresponding to the [M+H]+ ions of A) Main peptide fragment from a tryptic digest of native EeCentrocin 1, B) Synthesised fragment GWBrWBrR, and C) Calculated isotope distribution of [GWBrWBrR+H]+. The identical and distinctive distributions (A, B and C) of the singly charged isotopes indicate the presence of two Br-Trp residues in EeCentrocin 1.
