Table 1. Peptide fractions with corresponding retention times (min) obtained by preparative RP-HPLC, monoisotopic masses (Da) of the native peptides obtained by HR-MS, and the partial amino acid sequences obtained by Edman degradation analysis.
Peptide fraction | Retention time (min) | Mass (Da) | Amino acid sequence |
---|---|---|---|
Ee4635/Ee5024 | 31 | 4630.17/5019.38 | Seq 1: XGGKTXGKIKHAARKGVGYASRGCRIAGH |
Seq 2: XGHKLRSSXNKVKHAVKKGAGYASGACRVLGH | |||
Ee5922 | 32 | 5917.77 | Seq 3: XNPFKKIAHRXCYPKNE |
Seq 4: RXACTVVAQ | |||
Ee4835 | 38 | 4830.20 | Seq 5: GXXRRTVDKVRNAGRKVAGFASKACGALGH |
Seq 6: DIGKYCGYAHALN |