Fig 6. Lysis of Saccharomyces cerevisiae live cells in hypotonic media during incubation with soluble fraction of Aedes aegypti larvae guts.
The cells were incubated in 10 mM citrate-sodium phosphate buffer pH 7. Controls—Saccharomyces cerevisiae cells (10 CFUs/uL) in buffer; Gut Homogenates—S. cerevisiae cells (10 CFUs/uL) incubated with the soluble fraction of A. aegypti larval midguts (0.1 animal/uL) Gut Homogenates + Laminarin—S. cerevisiae cells (10 CFUs/uL) incubated with the soluble fraction of A. aegypti larval midguts (0.1 animal/uL) plus laminarin (1.7%, w/v). Figures are means ± SEM of 3 experiments with 3 samples obtained from 10 insects each.