Figure 2.

T80K GRS1 fails to support yeast cell growth. Haploid Δgrs1 yeast strains were transformed with a vector containing no insert (pRS315 Empty) or an insert to express wild-type or T80K GRS1 (equivalent to the T209K, s266 zebrafish mutation, T130K in human GARS). Cultures resulting from each transformation were spotted undiluted and diluted (1:10 and 1:100) on plates containing 0.1% 5-FOA. Experiments were performed using two independently generated T80K GRS1 expression constructs (A and B).