Figure 2.

Expression profile of proliferating and differentiating myoblasts derived from DM1 and control mouse models. Analysis of endogenous Acta1 (A), transgene (B) and endogenous Dmpk (C) mRNA levels by northern blotting. Each hybridization signal was normalized to that of 18S rRNA and then compared with the normalized value measured for the same (trans)gene in the GPS muscle from that particular DM1 mouse model. Data were obtained from two independent culture series per cell line, for which triplicate cultures per series were pooled and analyzed; bars represent mean + SEM. For profiling of endogenous Dmpk (C) in Tg26 samples RT-qPCR was used, because signals of transgenic DMPK and endogenous Dmpk overlapped on northern blot. Bars represent mean + SEM. Timing of differentiation on the x-axis refers to Figure 1.