Leydig cells in different regions of testicular parenchyma in pigs at 7, 90 and 180 days of age. Leydig cells near or under the tunica albuginea (A, D, G) and near the mediastinum (transitional region, C, F, I) are smaller in comparison to those in the intermediate region (B, E, H). TA, tunica albuginea; TR, transitional region; SC, seminiferous cords; ST, seminiferous tubules; L, Leydig cell (bar = 40 μm).