Figure 12.

T-REX exploits the on-demand precision targeting concept. Inert photocaged precursors delivered to cells selectively and covalently bind HaloTag fusion proteins (inset) and excess is washed away. Photouncaging driven by 365 nm light rapidly unleashes up to stoichiometric amounts of reactive lipid-derived signaling electrophiles (LDEs) from the HaloTag as a point source (inset, representative LDEs). Targeted LDE modifications on a specific POI in turn elicit gene-specific redox signaling on demand in the backdrop of an unperturbed cell. Reproduced from ref (19). Copyright 2015 American Chemical Society.