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Proximity-specific ribosome profiling. This deep sequencing-based method requires coexpression of spatially restricted (e.g., ER or mitochondrial membrane) biotin ligase BirA and ribosomes fused to its substrate, AVI tag. Pulsing of live cells with biotin enables biotinylation of proximal ribosomes. Enrichment of biotinylated ribosomes and subsequent deep sequencing of ribosome-protected mRNAs inform spatiotemporally precise message translation.
