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. 2016 Feb 23;138(11):3610–3622. doi: 10.1021/jacs.5b12608

Figure 5.

Figure 5

LUCIDs. Tethered TMP binding to DHFR (POI) enables BRET between the nano-Luc enzyme and Cy3 dye. Titration with methotrexate (blue pentagon) displaces TMP, thus preventing BRET.