Table 1.

List of primary antibodies for Western blotting, immunocytochemistry and immunohistochemistry

Primary antibodies	Company	Western blot dilution		Cell staining dilution
Host, protein (SwissProt name)		WCL	CM	In vitro	In vivo
Rabbit anti-α-actinin (ACTN4)	Epitomics	1:20,000	1:2,000
Rabbit anti-aquaporin 4(AQP4)	Sigma	N/A	N/A	N/A	1:10,000 (DAB)
Rabbit anti-Aldolase C (ALDOC)	GeneTex	1:20,000	1:3,000
Goat anti-ALDOC	Santa Cruz
Mouse IgG anti-αB-Crystallin (CRYAB)	Enzo Life Sciences	1:3,000	1:1,500		1:150 (IF)
Rabbit anti-apolipoprotein E (APOE)	Abcam	1:2,000	1:3,000		1:100 (IF)
Mouse IgG anti-β-actin (ACTB)	Sigma-Aldrich	1:70,000	1:3,000
Mouse IgG anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH)	GeneTex	1:20,000	1:2000
Rabbit anti-GFAP	Dako	1:500,000	1:10,000	1:600	1:20,000 (DAB)
Chicken anti-GFAP	Sigma-Aldrich	1:500,000	1:130,000		1:500 (IF)
Rabbit anti-glutamine synthetase (GLNA)*	Abcam	1:40,000	1:8,000		1:500 (IF)
Rabbit anti-lactate dehydrogenase (LDHB)	Cell Signaling	1:6,000	1:2,000
Rabbit anti-PEA15	GeneTex	1:10,000	1:1,000	1:200	1:200 (IF)
Rabbit anti-peroxiredoxin (PRDX1)	Enzo Life Sciences	1:10,000	1:2,000
Rabbit anti-superoxide dismutase (SODC)	Cell Signaling	1:7,000	N/A		1:100 (IF)
Rabbit anti-STAT3	GeneTex	1:200,000	1:10,000
Rabbit anti-transgelin (TAGL)
Rabbit anti-tropomyosin 4 (TPM4)	GeneTex	1:8,000	1:2,000
Mouse IgM anti-vimentin (VIME)	Sigma-Aldrich	1:15,000	1:1,000
Mouse IgG anti-vinculin (VINC)	Sigma-Aldrich	1:20,000	1:50,000
Rabbit anti-Y wha 14-3-3-ε (1433E)	Chemicon	1:10,000	1:6,000

^*Femto ECL chemiluminescence substrate was used for the detection of GLNA enabling higher antibody dilution to suppress background.

Pico ECL was used for all other antibodies.