Table 5.

Quantification of subcellular GFAP distribution patterns and cell permeability

Listed are mean percentages +/− SEM of GFAP-positive populations (GFAP+, black column) that were comprised of filament assembled (hatched) and disassembled (gray) signals and a small population of cells with far above average bright GFAP signals (yellow column). STAT3-CKO astrocytes had a considerable cell fraction with granular GFAP aggregates (light gray column). Populations were analyzed in cultures that were unstretched (n=5 mice each genotype), acutely stretched (5 and 30 min post-injury combined, n=10 CON and 5 STAT3-CKO mice) and at 48 h post-injury (n=5 mice each genotype).