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. Author manuscript; available in PMC: 2016 Mar 23.
Published in final edited form as: Cell Rep. 2016 Mar 10;14(11):2511–2518. doi: 10.1016/j.celrep.2016.02.059

Figure 2. Distinct localization patterns for the early and late melanin enzymes.

Figure 2

(A) The melanin gene cluster in A. fumigatus and the predicted localization of melanin enzymes in diverse fungal species. S: Secreted protein; C: Cytosolic protein; PM: Plasma membrane; Mit: Mitochondria; a: Ascomycetes; b: Basidiomycetes; *: melanin genes not arranged in a cluster; ?: the presence of an ortholog is uncertain.

(B) The expression and localization of Abr2 during development. [a] vegetative hypha; [b] young stalk; [c–d] mature stalk; [e–f] young conidiophore; [g] conidia.

(C) Localization of Alb1 in conidia.

(D) Localization of Alb1-GFP and the endosomal marker Rab5-mCherry, and a fluorescence intensity plot along a cellular axis indicated with a white line.

(E) Localization of Alb1-GFP and Mvp1-mCherry, and a fluorescence intensity plot showing co-localization.

Localization(F) and fluorescence intensity (G) of the early melanin enzyme wA-GFP in WT and mvp1Δ in A. nidulans.

Localization (H) and fluorescence intensity (I) of the late melanin enzyme yA-chRFP in WT and mvp1Δ. Scale bars in panels D–F: 2.5 μm.

See also Figure S2