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. Author manuscript; available in PMC: 2017 Mar 23.
Published in final edited form as: J Am Chem Soc. 2016 Mar 11;138(11):3639–3642. doi: 10.1021/jacs.6b00445

Figure 1.

Figure 1

(A) HPLC chromatogram showing formation of ADT 10 in the presence of different sulfur sources. Assays were carried out in the presence of 20 μM MjThi4, 500 μM ADP-ribose (ADPr) 2, 1 mM glycine, 400 μM ferrous ammonium sulfate (FeAS) and 0.8-1 mM of each of the sulfur compounds. Assays were quenched with 8 M GuHCl after 1 hour anaerobic incubation at 85 °C. (B) HPLC chromatogram showing conversion of NAD into ADT in the presence 100 μM MjThi4, 300 μM NAD, 1 mM glycine, 400 μM FeAS and 800 μM Na2S at 25 °C. (C) Overlaid UV-Vis spectra of the reaction product shown in panel A and an authentic sample of ADT prepared using ScTHI4. (D) Production of ADP-ribulose (ADPrl) 3 from ADPr 2 by MjThi4. Conditions were as follows; 500 μM MjThi4, 200 μM ADPr, 1 mM glycine, 1 mM FeAS and 800 μM Na2S inside a glovebox at 25 °C.