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. Author manuscript; available in PMC: 2016 Mar 23.
Published in final edited form as: Cell Rep. 2016 Mar 10;14(11):2587–2598. doi: 10.1016/j.celrep.2016.02.045

Figure 1. RBBP4 modulates TMZ sensitivity in GBM cells.

Figure 1

(A) Absolute levels of RBBP4 shRNA in GBM22 cells following treatment with either DMSO or TMZ. (B) Effect of RBBP4 siRNA, with and without TMZ treatment, on the primary neurosphere (NS) formation in GBM12 and GBM22 cells. (C) RBBP4 expression in T98G cells stably expressing shNT and 4 different RBBP4 shRNA lentiviral constructs (upper panel) and in vitro TMZ cytotoxicity CyQuant assay in the same cell constructs (lower panel). (D) Effect of control shNT and 4 different RBBP4 shRNA on RBBP4 levels in U138 GBM cells (upper panel) and in vitro cytotoxicity. Shown is the data from 3 independent experiments conducted in triplicate (error bar =S.E.M; * = p <0.05).