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. Author manuscript; available in PMC: 2016 Mar 23.
Published in final edited form as: Cell Rep. 2016 Mar 10;14(11):2587–2598. doi: 10.1016/j.celrep.2016.02.045

Figure 3. RBBP4 modulates repair of TMZ-induced DNA damage and regulates RAD51 expression level in GBM cells.

Figure 3

(A) Western blot depicting the time-course activation of DNA damage signaling following TMZ comparing T98G shNT and shRBBP4-3 clone (B) Nuclear p-H2AX foci in response to 72 hour exposure of T98G cells expressing shNT compared with shRBBP4-3 clone (upper panel), while the lower panel shows the fraction of cells with mean γ-H2AX foci of 3 experiments conducted in triplicate (C) Effect of RBBP4 disruption by shRNA clone shRBBP4 on PARP inhibitor ABT-888 sensitization in T98G GBM cells measured in a CyQuant assay (D) Real time PCR showing RAD51 and BRCA1 expression in T98G-shNT cells compared to T98G-shRBBP4. (E) Real time PCR evaluating RAD51 expression comparing T98G-shRBBP4_pCDNA3 with the RBBP4 reconstituted T98G-shRBBP4_R1 and _R4 cells (F) CyQuant evaluation of ABT-888 sensitivity after RBBP4 reconstitution in T98G-shRBBP4 clone compared with the T98G-shRBBP4_pcDNA3 and T98G-shNT cells Error bar = S.E.M.; * = p<0.05).