Figure 1. Experimental design and brain regions activated by tactile Braille reading.

(A) The study consisted of two identical MRI sessions performed before and after an intensive 9-month tactile Braille course. In the fMRI experiment (B), subjects viewed visual words written in the regular alphabet and Braille words displayed on a screen (visual Braille) and touched tactile Braille words. As a control, they viewed strings of hash signs and meaningless pseudo-Braille dots and touched meaningless pseudo-Braille dots. The experiment used a block design (C); after each block, the subjects lifted their fingers from the table on which the tactile stimuli were presented. A new board with tactile stimuli was then placed on the table, the subjects put their fingers down, and a new block began. (D) Compared to the tactile control, tactile reading after the course evoked activations in the visual word form area (VWFA), the lateral occipital area and other areas (Tables 1–3). Similar areas were activated when we computed (E) an interaction between tactile Braille/tactile control stimuli and the before- and after-course time points and for (F) an interaction between tactile Braille/tactile control vs. visual reading/visual control stimuli and the before- and after-course time points. The latter confirmed that the increase in visual cortex activation after the course was specific for tactile reading. (G) When we modeled the modulation of fMRI responses to tactile Braille by the subjects’ reading speed, the only significant whole-brain correlate with tactile reading proficiency was found in the ventral visual system (k=103 voxels). Voxel-wise thresholds: (D) p<0.001; (E–G) p<0.005; Cluster-wise thresholds: (D–F) p<0.05; (G) uncorrected, k=100 voxels. For control fMRI experiment, see Figure 1—figure supplement 1 for procedures and Figure 1—figure supplement 2 for results. For supplementary fMRI results, see Figure 1—figure supplement 3.

DOI: http://dx.doi.org/10.7554/eLife.10762.003

Figure 1—figure supplement 1. Control experiment stimuli and procedures.

(A) In the control imagery experiment, subjects were asked to read or imagine words in the regular alphabet or in visual and tactile Braille or to touch or imagine everyday objects. (B) Similar to the Main experiment (Figure 1C), stimuli in the control experiment were presented in blocks. In each block, after a sound indicating they should lift their fingers from the fiberglass table, subjects heard an auditory instruction informing them about the next task (read/touch or imagine). After 8–12 s, a sound signaled them to put their fingers down. Subjects began to read/touch/imagine after 4–7 s, when they heard the name of the stimulus to be read/touched or imagined. (C) Blocks in the main experiment consisted of 8 stimuli: words, control stimuli or fixation dots in the case of tactile conditions. Each stimulus was presented for 3500 ms and was followed by a 500 ms fixation dot, which was accompanied with a metronome tick indicating the change of the stimulus for the tactile conditions. (D) Blocks in the control imagery experiment consisted of 6 stimuli. After a 500 ms fixation dot, an auditory recording of the name of the subsequent stimulus was presented, either with (read/touch task) or without the stimulus (imagery task). For object stimuli, the block duration was the same, but only one stimulus was presented per block.

DOI: http://dx.doi.org/10.7554/eLife.10762.007

Figure 1—figure supplement 2. Main results of the control experiment.

(A) Imagining Tactile Braille vs. rest elicited activity in motor/somatosensory areas, the cerebellum and left occipital areas, including the VWFA. Similar results were found for (B) Visual Braille imagery. However, (C) Tactile Braille imagery more strongly activated the somatosensory and motor areas and mesial frontal regions. (D) In addition to the parietal and sensory-motor activations, touching objects vs. rest resulted in activity in lateral occipital areas bilaterally. Thresholds: A,B p = 0.001 voxel-wise, p=0.05 cluster-wise; C, D p=0.005 voxel-wise, p=0.05 cluster wise.

DOI: http://dx.doi.org/10.7554/eLife.10762.008

Figure 1—figure supplement 3. Supplementary fMRI results.

(A) Visual word reading contrasted with the control task before the course activated left ventral occipital areas, including the VWFA and the middle occipital gyrus. (B) Visual Braille reading vs. control before the course elicited robust activation in occipital areas predominantly on the left side, including the VWFA and the inferior and middle occipital gyrus. Other activations included the left inferior and middle frontal gyri, the precuneus, the middle temporal gyrus and the left STS. (C) For visual Braille reading, the only increases in activation observed after the course were in the default mode network (mesial frontal cortex and bilateral temporal and parietal regions). (D) Similar to Braille word reading speed (Figure 1G), single-letter recognition speed modulated the activity for tactile Braille reading in the left occipital areas, including V1 and the fusiform gyrus (VWFA). Thresholds: A-C p=0.005 voxel-wise, p=0.05 cluster-wise; D p = 0.005 voxel-wise, uncorrected for cluster size.

DOI: http://dx.doi.org/10.7554/eLife.10762.009